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Product Info Summary
| SKU: | A02786-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-VRL1/TRPV2 Antibody Picoband™
SKU/Catalog Number
A02786-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VRL1/TRPV2 Antibody Picoband™ catalog # A02786-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VRL1/TRPV2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02786-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human VRL1/TRPV2 recombinant protein (Position: S7-K390).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02786-3 is reactive to Trpv2 in Human, Mouse, Rat
Applications
A02786-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
85.965kDa
Background of VRL1
TRPV2 (Transient Receptor Potential Cation Channel Subfamily V Member 2), also known as VRL1, is a protein that, in humans, is encoded by the TRPV1 gene. The International Radiation Hybrid Mapping Consortium mapped the TRPV2 gene to chromosome 17. This gene encodes an ion channel that is activated by high temperatures above 52C. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is though that in other tissues the channel may be activated by stimuli other than heat.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VRL1/TRPV2 using anti-VRL1/TRPV2 antibody (A02786-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRL1/TRPV2 antigen affinity purified polyclonal antibody (Catalog # A02786-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRL1/TRPV2 at approximately 95KD. The expected band size for VRL1/TRPV2 is at 95KD.
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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-VRL1/TRPV2 antibody (A02786-3).
Overlay histogram showing HL-60 cells stained with A02786-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VRL1/TRPV2 Antibody (A02786-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Trpv2 (Source: Uniprot.org, NCBI)
Gene Name
Trpv2
Full Name
Transient receptor potential cation channel subfamily V member 2
Weight
85.965kDa
Superfamily
transient receptor (TC 1.A.4) family
Alternative Names
Osm-9-like TRP channel 2; transient receptor potential cation channel, subfamily V, member 2; TrpV2; Vanilloid receptor-like protein 1; VRL-1MGC12549; VRL1transient receptor potential cation channel subfamily V member 2; VRLOTRPC2 Trpv2|GRC, OTR, OTRPC2, V, VR, VRL-1, Vrl1|transient receptor potential cation channel, subfamily V, member 2|transient receptor potential cation channel subfamily V member 2|growth factor regulated channel|growth factor-regulated calcium channel|osm-9-like TRP channel 2|vanilloid receptor-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Trpv2, check out the Trpv2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Trpv2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VRL1/TRPV2 Antibody Picoband™ (A02786-3)
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No publications found for A02786-3
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